Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
Print this page Email this page Users Online: 1006
REVIEW ARTICLE
Year : 2014  |  Volume : 3  |  Issue : 1  |  Page : 5-7

High-dose hook effect


1 Department of Medical Endocrinology and Metabolism, Narayana Medical College and Superspeciality Hospital, Nellore, Andhra Pradesh, India
2 Department of Medicine, Sri Venkateswara Medical College, Tirupati, Andhra Pradesh, India

Correspondence Address:
Rajendra Prasad Namburi
Narayana Medical College and Superspeciality Hospital, Nellore, Andhra Pradesh - 524 003
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2277-8632.128412

Rights and Permissions

High-dose effect is a common phenomenon documented in various immunological and serological assays, which results in false-negative results. Due to limited amounts of reagent antibodies in the coated wells and extremely high concentrations of analyte in the sample leads to decrease in the formation of antigen-antibody complex and ultimately resulting in false low values of the analyte. High-dose hook effect has important medical implications, and simple methods of sample dilution helps in preventing false low concentrations.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed5641    
    Printed109    
    Emailed1    
    PDF Downloaded689    
    Comments [Add]    
    Cited by others 6    

Recommend this journal