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ORIGINAL ARTICLE
Year : 2020  |  Volume : 9  |  Issue : 1  |  Page : 52-56

Differences in antimicrobial activity of 0.2% Chlorhexidine mouthwash and a Herbal mouth rinse formulation—A salivary bacterial counts study


1 Department of Periodontology, P.M. Nadagouda Memorial Dental College and Hospital, Bagalkot, Karnataka, India
2 Department of Periodontology, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India
3 Consultant Periodontist, Bangalore, Karnataka, India
4 Consultant Periodontist, Pune, Maharashtra, India
5 Department of Periodontology, MNR Dental College, Sangareddy, Telangana, India
6 Department of Oral pathology, Subbaiah Institute of Dental Sciences, Shimoga, Karnataka, India

Correspondence Address:
Dr. C Naresh Kumar
Department of Periodontology, Vishnu Dental College, Bhimavaram
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/JDRNTRUHS.JDRNTRUHS_66_19

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Background: Chlorhexidine gluconate (CHX) mouthwash has earned eponym of gold standard to treat or prevent periodontal disease. However, it has been reported to have local side-effects on long-term use. To explore a herbal alternative, the present study was carried out to evaluate the clinical antimicrobial activity of two mouth rinses. One containing 0.2% CHX and other containing herbal mouth rinse (HM). Methods: This randomized, crossover salivary bacterial counts, was carried out on a sample of 10 healthy volunteers rinsed for 1 minute with 0.2% CHX and for 1 minute with HM. Saliva samples were obtained before rinsing, and after 5 min, and 1, 3, 5 and 7 h. These samples were cultured both aerobically and anaerobically. Percentages of survival, in regard to baseline, were calculated for each time point. Results: Results were available both for aerobic and anaerobic salivary bacteria. Statistically significant difference is observed between Herbal and 0.2% CHX mouth wash at 1 hr (P < 0.05), 3 hrs (P < 0.01), 5 hrs (P < 0.01) as well as at 7 hrs (P < 0.001) and HM and 0.2% CHX mouth wash at 5 minutes after using mouth wash (P < 0.05), 1 hr (P < 0.05), 3 hrs (P < 0.01), 5 hrs (P < 0.01) as well as at 7 hrs (P < 0.001). Conclusion: Based on the results, this clinical study demonstrated that significant reduction of microbial activity was observed in 0.2% CHX when compared with HM containing bibhitaka, nagavali and salvodora persica as main ingredients.


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