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ORIGINAL ARTICLE
Year : 2013  |  Volume : 2  |  Issue : 1  |  Page : 42-46

Cytotoxic evaluation of two chlorine-releasing irrigating solutions on cultured human periodontal ligament fibroblasts


1 Department of Conservative Dentistry and Endodontics, Sibar Institute of Dental Sciences, Takkellapadu, Guntur, India
2 Department of Oral and Maxillofacial Pathology, Sibar Institute of Dental Sciences, Takkellapadu, Guntur, India
3 Department of Oral and Maxillofacial Pathology, Ragas Dental College, Chennai, India

Correspondence Address:
Nagesh Bolla
Department of Conservative Dentistry and Endodontics, Sibar Institute of Dental Sciences, Takkellapadu, Guntur
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2277-8632.108512

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Objective: Endodontic irrigants solutions with anti-bacterial activity have been used in treatment of teeth with infected root canals; however, these solutions can irritate periapical tissues. The objective of this study was to evaluate the cytotoxicity of two chlorine-releasing irrigating solutions on cultured human periodontal ligament fibroblasts. Materials and Methods: Periodontal ligament fibroblasts cells were cultured from orthodontically extracted premolars. The passage number between 3-6 was taken for further experiments. 2.5% sodium hypochlorite and 0.6% sodium dichloroisocyanuarate are the irrigating solution used for testing their cytotoxicity. The viability of cells after treating with irrigating solutions was evaluated by trypan blue dye exclusion. Results: Results showed that 0.6% NaDCC solution was less cytotoxic on cultured human periodontal ligament fibroblasts compared with 2.5% NaOCl at 2 min, 30 min, and 1 hr. Conclusion: This study suggested that sodium dichloroisocyanuarate is less cytotoxic than 2.5% NaOCl, suggesting its potential for use as root canal irrigant.


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