Journal of Dr. NTR University of Health Sciences

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 4  |  Issue : 2  |  Page : 112--116

Prevalence of Rotavirus diarrhea among under-5 hospitalized children in a Government tertiary hospital, Tirupati


Manohar Badur1, Naramalli Madhavi Latha2, Panabaka Ravi Kumar1, Shankar Reddy Dudala3, Shabbir Ali Shaik1, Gagandeep Kang4, Cheri Naveen Kumar1,  
1 Department of Pediatrics, Sri Venkateswara Medical College, Tirupati, Andhra Pradesh, India
2 Department of Bio-chemistry, Sri Venkateswara Medical College, Tirupati, Andhra Pradesh, India
3 Department of Community Medicine, Sri Venkateswara Medical College, Tirupati, Andhra Pradesh, India
4 Department of Gastrointestinal Sciences, Christian Medical College, Vellore, Tamil Nadu, India

Correspondence Address:
Dr. Manohar Badur
Department of Pediatrics, Sri Venkateswara Medical College and Sri Venkateswara Ramnarain Ruia (SVRR) Government General Hospital, Tirupati - 517 507, Andhra Pradesh
India

Abstract

Context: Rotavirus is the most common cause of severe diarrhea requiring hospitalization among infants and young children worldwide. The prevalence of rotavirus diarrhea in India has been found to vary in the range 5-71% in hospitalized children aged under 5 years with acute gastroenteritis. The seasonal variation of rotavirus diarrhea in India varies across different geographical regions, with high incidence in the winter months at low relative humidity in northern India. Aim: This study aimed to estimate the prevalence of rotavirus diarrhea among hospitalized children aged under 5 years and to learn about the genotypic distribution of rotaviruses causing diarrhea. Settings and Design: Study design: hospital-based cross-sectional study. Study setting: the pediatrics department of a tertiary care Government hospital, Tirupati, India. Study period: September 20, 2012-September 19, 2013. Materials and Methods: Study units: children under 5 years of age presenting with diarrhea. Stool specimens from all hospitalized children aged under 5 years who had presented with acute watery diarrhea were collected and tested for rotavirus by the enzyme-linked immunosorbent assay (ELISA). Positive samples were tested for G and P typing by the reverse transcription-polymerase chain reaction (RT-PCR) technique. Statistical Analysis Used: Percentage and chi-square analysis. Results: Among the study sample, 68.7% of children were in the age group between 1-12 months and 25.6% children showed positive result for rotavirus by ELISA. Of the rotavirus positives, 50% were G1P8 viruses. Conclusion: Rotavirus is an important cause of diarrhea in hospitalized children.



How to cite this article:
Badur M, Latha NM, Kumar PR, Dudala SR, Shaik SA, Kang G, Kumar CN. Prevalence of Rotavirus diarrhea among under-5 hospitalized children in a Government tertiary hospital, Tirupati.J NTR Univ Health Sci 2015;4:112-116


How to cite this URL:
Badur M, Latha NM, Kumar PR, Dudala SR, Shaik SA, Kang G, Kumar CN. Prevalence of Rotavirus diarrhea among under-5 hospitalized children in a Government tertiary hospital, Tirupati. J NTR Univ Health Sci [serial online] 2015 [cited 2021 Oct 28 ];4:112-116
Available from: https://www.jdrntruhs.org/text.asp?2015/4/2/112/158589


Full Text

 INTRODUCTION



Rotavirus is the most common cause of severe diarrhea requiring hospitalization among infants and young children worldwide. [1] Very few studies on the prevalence of rotavirus among children have been conducted in Andhra Pradesh. Rotavirus infections spread easily through multiple modes of transmission. Rotavirus infections also spread in settings where many children are together, such as day-care centers and nurseries. Marked seasonality is seen in temperate or cooler climates, where outbreaks usually occur in the winter and early spring, between about November and April. Rotaviruses are classified based on the most abundant protein VP6, into groups A-G, with groups A-C infecting humans. Among them, Group A rotavirus is the most important human pathogen. [2] Diarrhea caused by rotaviruses may be due to impaired sodium and glucose absorption, as damaged cells on villi are replaced by nonabsorbing immature crypt cells. [3]

Exposure to infection occurs in early life. By the age of 3 years, 90% of children have serum antibodies against one or more types of rotavirus, indicating high levels of exposure. Several studies on rotavirus epidemiology have been carried out in different parts of India. Previous studies in the Indian Rotavirus Strain Surveillance Network have confirmed that rotavirus accounts for 39% of acute diarrheal hospitalizations. [4] This study aimed to identify the proportion of children with acute gastroenteritis infected with rotavirus through systematic sampling over a 1-year period in a tertiary care Government hospital in Andhra Pradesh.

 MATERIALS AND METHODS



The study was conducted at a Government tertiary care hospital in Tirupati, Chittoor district, Andhra Pradesh, India from September 20, 2012 to September 19, 2013. This study included all hospitalized children of aged under 5 years who had presented with acute watery diarrhea. Informed consent was obtained for each child from the respective parent/guardian after explaining the purpose of the study. A case of diarrhea was defined as increased stool frequency, compared with the usual pattern occurring in a child aged under 5 years for whom the parents sought care for treatment of diarrhea. [3] Clinical details including age, sex, duration of illness, number of stools, associated vomiting and fever, degree of dehydration, and concomitant illness were recorded on a standardized case reporting form. This study is part of an Indian Council of Medical Research (ICMR)-funded project for the National Hospital Based Rotavirus Surveillance Network. Our Government tertiary care hospital in Tirupati is one of the study sites of the multicentric project, and we obtained Institutional Ethical Committee approval prior to commencement of the study.

Stool specimens from all hospitalized children aged under 5 years who had presented with acute watery diarrhea were collected and stored in the refrigerator at 4°C and later transported to the base hospital in iceboxes. All the stool samples were sent to the testing laboratory at the Christian Medical College (CMC), Vellore, Tamil Nadu at 4°C. Samples were tested for the presence of rotavirus using a commercially available antigen detection apparatus, the enzyme-linked immunosorbent assay (ELISA) (Premier TM Rotaclone ® , Meridian Biosciences, Cincinnati, Ohio, USA), as per kit protocol. Samples showing an optical density value of ≥0.150 were reported as positive. An internal control was included in all runs, and the run was repeated if the internal control did not fall in the expected range.

Genotyping was performed on the antigen-positive samples. Ribonucleic acid (RNA) was extracted using the QIAamp Viral RNA Mini Kit (Qiagen N.V., Venlo, Netherlands). Complementary deoxyribonucleic acid (DNA) was synthesized using random primers [Pd(N)6 hexamers; Pharmacia Biotech, Amersham Pharmacia Biotech, Piscataway, New Jersey, USA] and 400 units of Moloney murine leukemia virus reverse transcriptase (Invitrogen Life Technologies, Thermo Fisher Scientific, Waltham, Massachusetts, USA), and was used as the template for VP7 and VP4 (G and P) typing in PCRs using published oligonucleotide primers and protocols to detect the VP7 genotypes G1, G2, G3, G4, G8, G9, G10, and G12, and the VP4 genotypes P[4], P[6], P[8], P[9], P[10], and P[11]. [4]

Data were collected and entered into Microsoft Excel (Microsoft Corporation, Redmond, Washington, USA) software that were later analyzed using SPSS version 16.0 (IBM Corporation, Armonk, New York, USA). Appropriate statistical tests, i.e., percentage calculation and chi-square analysis, were performed.

 RESULTS



For the 1-year period, a total of 187 children were included in the study group. Among the study subjects, a majority of them were male children [107 (57.2%)], and 80 (42.8%) were female children. The stool samples of 48 (25.67%) children were positive for rotavirus by ELISA [Table 1]. Among 187 cases, a majority [110 (58.8%)] were in the age group of 1-12 months. Similarly, out of 48 ELISA-positive cases, 33 (17.7%) were found to be in the same age group. There were no rotavirus-infected cases among neonates and in the age group of 48-60 months [Table 2]. Applying chi-square analysis, it was found that there was no statistical significant difference in ELISA reactivity between different age groups of cases. Of 48 ELISA positives, 24 (50%) belonged to the G1P8 type [Table 3]. Out of the 48 ELISA-positive cases, 27 belong to the G1 type, followed by G12 and G2 [Table 4]. Most (32) of the rotaviruses belonged to the P8 type [Table 5].{Table 1}{Table 2}{Table 3}{Table 4}{Table 5}

 DISCUSSION



In the present study, it was found that 25.66% of the children aged under 5 years, hospitalized with the complaint of diarrhea are due to rotavirus. The Indian Rotavirus Strain Surveillance Network carried out a multicentric study in seven different regions of India and reported that rotavirus was detected in the stools of 39% children aged under 5 years. [4],[5] Studies in other parts of Asia have shown a much higher prevalence rate, perhaps due to the absence or lower rates of other causes of acute gastroenteritis. [6] A few studies done in Indian outpatient facilities and in the community revealed that 30% of cases were under 6 months of age. [7],[8]

In Chandigarh, rotavirus was detected in 16-19% of instances of acute gastroenteritis in children under 5 years of age, [9],[10],[11] while in Aligarh it was detected in 19% of cases of acute diarrhea. [12] In the eastern states of India and in Pune, rotavirus was detected in 28-30% of children aged under 5 years with acute diarrhea. [13],[14] In Kolkata, the incidence of rotavirus-associated diarrhea varied 5-22%, [15],[16] but in Manipur, the incidence was as high as 41%. [17] However, it is important to note that these studies did not use similar methods and therefore a direct comparison of results is difficult.

Regarding seasonality, some studies in India have found no association between rotavirus infection and the time of year. [8],[18] Other studies have observed an increase in rotavirus-associated diarrhea during the winter months, October-February, throughout the country. [19],[20],[21] Rotavirus was markedly seasonal in northern India but was less seasonal in southern locations with a more tropical climate. [4],[22],[23] In the present study, although the number of cases increased from December to April, there was uneven distribution throughout the year [Figure 1].{Figure 1}

In the present study, 56.25% of the rotaviruses were of G1P [8] genotype, followed by G12P [6] (14.5%) and G12 [P8] (12.5%). In a study from Kolkata, the predominant genotype was G1P [8] (20%), followed by G2P [4] (15%) and G4P [8] (6%). A number of uncommon genotypes, G1P [4] (4%), G2P [8] (2.5%), G2P [6] (0.6%), G4P [4] (2.5%), and G4P [6] (1.25%), were also observed. [24] A rotavirus strain not common in India, G4P [8], was reported in children with acute diarrhea in another study at Kolkata. [25]

In another study from Vellore on 100 rotavirus strains, the commonest G types seen were G1, G4, G2, G9, G3, and G8, in order of frequency, and the P types were P[4], P[8], and P[6]; the most common G:P combinations were G1P[8], G1P[4], G2 P[4], and G4 P[8]. [26] A study done in Kerala also showed that G1P[8] is the most common genotype, followed by G9P[8]. [27] The tracking of strains is important to understand the epidemiology of the disease and to monitor changes following the introduction of vaccine.

There were a few limitations of present study. As this study is hospital-based, the prevalence of rotavirus might have been different from the actual prevalence in the community. The incidence of the disease could not be calculated in this study.

This study has made it clear that one-fourth of the diarrheal disorders among children aged under 5 years are due to rotavirus, which calls for stringent preventive measures in terms of compulsory vaccination against rotavirus. Due importance should also be given to the personal hygiene and handwashing practices of family members.

References

1Centers for Disease Control and Prevention (CDC). Rotavirus surveillance--worldwide, 2001-2008. MMWR Morb Mortal Wkly Rep 2008;57:1255-7.
2Kang G, Kelkar SD, Chitambar SD, Ray P, Naik T. Epidemiological profile of rotaviral infection in India: Challenges for the 21 st Century. J Infect Dis 2005;192(Suppl 1):S120-6.
3Mustafa NS, Elhag WI. Diagnosis of rotavirus gastroenteritis by a latex agglutination test in Khartoum State, Sudan. J Pharm Biomed Sci 2013;33:1594-8.
4Kang G, Arora R, Chitambar SD, Deshpande J, Gupte MD, Kulkarni M, et al.; Indian Rotavirus Strain Surveillance Network. Multicenter, hospital-based surveillance of rotavirus disease and strains among Indian children aged <5 years. J Infect Dis 2009;200:S147-53.
5Katoch VM. Data for action: The Indian Rotavirus Surveillance Network. Vaccine 2014; 32(Supplement 1): A1.
6Nelson EA, Bresee JS, Parashar UD, Widdowson MA, Glass RI; Asian Rotavirus Surveillance Network. Rotavirus epidemiology: The Asian rotavirus surveillance network. Vaccine 2008;26:3192-6.
7Kelkar SD, Purohit SG, Boralkar AN, Verma SP. Prevalence of rotavirus diarrhea among outpatients and hospitalized patients: A comparison. Southeast Asian J Trop Med Public Health 2001;32:494-9.
8Banerjee I, Ramani S, Primrose B, Moses P, Iturriza- Gomara M, Gray JJ, et al. Comparative study of the epidemiology of rotavirus in children from a community based birth cohort and a hospital in South India. J Clin Microbiol 2006;44:2468-74.
9Broor S, Singh V, Venkateshwarlu, Gautam S, Mehta S, Mehta SK. Rotavirus diarrhoea in children in Chandigarh, India. J Diarrhoeal Dis Res 1985;3:158-61.
10Singh V, Broor S, Mehta S, Mehta SK. Molecular epidemiology of human rotavirus infections in Chandigarh (India). Indian J Med Res 1990;91:9-14.
11Sharma P, Sehgal R, Ganguly NK, Vaidyanathan U, Walia BN. Serogroups of rotavirus in north India. J Trop Pediatr 1994;40:58-9.
12Malik A, Rattan A, Malik MA, Shukla I. Rotavirus diarrhoea of infancy and childhood in a north Indian town-epidemiological aspects. J Trop Pediatr 1987;33:243-5.
13Jain V, Das BK, Bhan MK, Glass RI, Gentsch JR; Indian Strain Surveillance Collaborating Laboratories. Great diversity of group A rotavirus strains and high prevalence of mixed rotavirus infections in India. J Clin Microbiol 2001;39:3524-9.
14Kelkar SD, Purohit SG, Simha KV. Prevalence of rotavirus diarrhoea among hospitalized children in Pune, India. Indian J Med Res 1999;109:131-5.
15Ghosh AR, Nair GB, Dutta P, Pal SC, Sen D. Acute diarrhoeal diseases in infants aged below six months in hospital in Calcutta, India: An aetiological study. Trans R Soc Trop Med Hyg 1991;85:796-8.
16Saha MR, Bhattacharya SK, Mukherjee AK, Chakraborty BN, Pal SC. Prevalence of rotavirus infection among neonates in Calcutta. Indian J Med Res 1984;80:620-2.
17Jain V, Parashar UD, Glass RI, Bhan MK. Epidemiology of rotavirus in India. Indian J Pediatr 2001;68:855-62.
18Mukherjee A, Chattopadhyay S, Bagchi P, Dutta D, Singh NB, Arora R, et al. Surveillance and molecular characterization of rotavirus strains circulating in Manipur, north-eastern India: Increasing prevalence of emerging G12 strains. Infect Genet Evol 2010;10:311-20.
19Bahl R, Ray P, Subodh S, Shambharkar P, Saxena M, Parashar U, et al.; Delhi Rotavirus Study Group. Incidence of severe rotavirus diarrhea in New Delhi, India, and G and P types of the infecting rotavirus strains. J Infect Dis 2005;192(Suppl 1):S114-9.
20Kahn G, Fitzwater S, Tate J, Kang G, Ganguly N, Nair G, et al. Epidemiology and Prospects for Prevention of Rotavirus Disease in India. Indian Pediatrics 2012; 49: 469.
21Saravanan P, Ananthan S, Ananthasubramanian M. Rotavirus infection among infants and young children in Chennai, South India. Indian J Med Microbiol 2004;22:212-21.
22Ray P, Sharma S, Agarwal RK, Longmei K, Gentsch JR, Paul VK, et al. First detection of G12 rotaviruses in newborns with neonatal rotavirus infection at all India Institute of Medical Sciences, New Delhi, India. J Clin Microbiol 2007;45:3824-7.
23Phukan AC, Patgiri DK, Mahanta J. Rotavirus associated acute diarrhoea in hospitalized children in Dibrugarh, North-east India. Indian J Pathol Microbiol 2003;46:274-8.
24Das S, Sen A, Uma G, Varghese V, Chaudhuri S, Bhattacharya SK, et al. Genomic diversity of group A rotavirus strains infecting humans in eastern India. J Clin Microbiol 2002;40:146-9.
25Khetawat D, Dutta P, Gupta S, Chakrabarti S. Emergence of rotavirus G4P8 strain among children suffering from watery diarrhoea in Calcutta, India. Intervirology 2001;44:306-10.
26Brown DW, Mathan MM, Mathew M, Martin R, Beards GM, Mathan VI. Rotavirus epidemiology in Vellore, South India: Group, subgroup, serotype, and electrophoretype. J Clin Microbiol 1988;26:2410-4.
27Mathew MA, Paulose A, Chitralekha S, Nair MK, Kang G, Kilgore P. Prevalence of rotavirus diarrhea among hospitalized under-five children. Indian Pediatr 2014;51:27-31.